P34: PRINTABILITY & CYTOCOMPATIBILITY TESTING OF XANTHAN GUM FOR EXTRUSION-BASED 3D BIOPRINTING IN WOUND HEALING

Abstract Introduction Bioprinting facilitates highly-specific deposition of skin cells and biomaterials. Skin bioprinting is still limited by prolonged printing processes, poor mechanical properties incomplete complexities. An important barrier identifying an ideal hydrogel for – one with a good balance between viable producing robust scaffolds. Herein, three hydrogels were tested potential use as bio-inks. Method A pneumatic-extrusion Inkredible 3D bioprinter was used. Initially, different concentrations Alginate (reference), XG Alginate/XG mixture quantitatively evaluated printability calculating aspect ratio, square cylinder height. The best two printable each selected to undergo hDF viability testing assess the effect process (Nozzle diameter viscosity). concentration group cell then underwent 14-day cytocompatibility period using Live/dead staining, metabolic activity, dsDNA collagen quantification. Result revealed remarkable ability build shapes (XG(6%) 27G nozzle-10.0mm±0.2 height). XG(6%), Alginate/XG(4%/10%) Alginate(6%) identified testing. XG(6%) had better day 1 (87.3%±2.9; 87.6%±1.5, respectively) than Alginate(6%)(74%±2.65). All gradually improving viability. Cell proliferation remained comparable amongst all hydrogels. Moreover, production confirmed in constructs. Conclusion Bioprinted cytocompatible further optimisation required prolong its degradability optimise application bioprinting. Abbrev XG–Xanthan gum; hDF–human Dermal Fibroblasts Take-home message There no purpose updated systematic review should be carried out current available